Active Ingredient: Doxycycline
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Ishikawa et al.
However, although minocycline is recommended for the aforementioned skin disorders, current therapy has moved towards the use of doxycycline, which has a similar efficacy for the treatment of these conditions with a reduced incidence of adverse effects, such as drug hypersensitivity syndrome, hyperpigmentation, and dizziness, frequently associated with minocycline therapy.
For this reason, aim of our study was to investigate, in vitro, if low doxycycline doses could be able to downregulate some of the cytokines involved in the pathogenesis of inflammatory skin diseases.
Materials and Methods 2. From that, we extrapolated the theoretical doxycycline blood concentrations of 0. To evaluate the protective or restoring doxycycline properties on LPS-stimulated HaCaT cells, three experimental settings were used, differing in the timing of doxycycline treatment in respect to the insult induced by LPS: pretreatment, concomitant, and posttreatment.
For the pretreatment setting, cells were preincubated with doxycycline for 24 hours before receiving LPS; thereafter, 24 hours later, cells were harvested and mRNA extraction was performed: this was 48 hours after doxycycline incubation and 24 hours after LPS stimulation.
In the concomitant setting, LPS and doxycycline were simultaneously added to the cells and mRNA was extracted 24 hours later. Lastly, for the posttreatment assessment, cells were stimulated with LPS for 24 hours and thereafter doxycycline was added; cells were incubated for further 24 hours and RNA extraction was performed at 48 hours Figure 1.
Cell viability was determined with the Trypan blue method after all treatments. Does the Deduct Apply: Some drugs do not require that the deductible is met before you receive coverage.
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